HPLC

Accessible and reliable HPLC chromatography

HPLC chromatography is an analytical technique widely used to separate and identify the constituents of a liquid mixture. It relies on the circulation of a solvent at high pressure through a column containing a stationary phase, allowing molecules to be differentiated based on their physicochemical properties. This method is recognized for its precision, sensitivity, and ability to handle a wide variety of samples.

Rewake offers a selection of tested, ready-to-use equipment to make this technology accessible to laboratories with varying budgetary and operational constraints. The available systems meet standard chromatography analysis requirements, whether for quality control, applied research, or analytical development. By utilizing refurbished laboratory equipment, teams benefit from a reliable solution that is immediately operational and compatible with current standards, all while limiting the costs and lead times associated with acquiring new equipment.

What is an HPLC system used for in a laboratory?

An HPLC system is designed to separate, identify, and quantify the various constituents of a complex mixture. In practice, it produces a chromatogram that highlights the compounds present, their relative concentration, and their retention time. This method is based on the principle of separation between a mobile phase (liquid) and a stationary phase (usually a porous material like silica), in which molecules migrate at different speeds depending on their chemical properties.

HPLC equipment is essential in many analytical protocols where precision and reproducibility are crucial. It is commonly used for:

• Assaying active substances in drugs
• Detecting contaminants in environmental samples
• Analyzing organic constituents in complex formulations

Thanks to their compatibility with various types of detectors (UV, fluorescence, mass spectrometry), HPLC systems allow for great flexibility in applications. They provide a high-performance solution for the chromatographic separation of polar or non-polar compounds, even at low concentrations.

Fields of application for HPLC chromatography

HPLC chromatography is used in a multitude of sectors where the analysis of chemical substances is essential. Its high level of precision, combined with its ability to separate complex compounds, makes it an indispensable method in several scientific and industrial fields:

• Pharmaceutical industry: quality control of active ingredients, purity verification, assaying of excipients or residual impurities.
• Cosmetics: stability analysis of formulations, detection of preservatives or sensitive molecules.
• Agri-food: detection of contaminants, additives, or pesticide residues in food.
• Environment: measuring pollutants in soil, water, or ambient air.
• Analytical chemistry: characterization of complex mixtures, research into the properties of molecules and their behavior in the liquid phase.

Thanks to its many coupling possibilities (UV, MS, diode array, etc.), HPLC is a reference technique for quantitative and qualitative analysis. It is used for both research and regulatory analysis, with strict requirements for reproducibility and sensitivity.

HPLC equipment offered by Rewake

Rewake's HPLC collection brings together equipment designed to meet the requirements of analytical laboratories, whether for research, quality control, or regulatory analysis. The systems offered cover the essential needs of high-performance liquid chromatography, with configurations adapted to different levels of analytical complexity.

Laboratories can find the following in this collection:

• Complete refurbished HPLC systems, including high-pressure pumps, injectors, and control modules.
• HPLC columns adapted for the separation of polar or non-polar compounds, depending on the stationary phase used.
• UV detectors, diode array detectors, or detectors compatible with mass spectrometer coupling.
• Manual or automatic injectors ensuring precise and reproducible injection.

Each piece of equipment is cleaned, checked, and tested before being put on sale to ensure reliable operation from the moment of installation. Refurbishment allows laboratories to access high-performance equipment while controlling their budget, without compromising on analytical quality or the robustness of the apparatus.

Why choose a refurbished HPLC from Rewake?

Rewake is committed to offering refurbished laboratory equipment that meets the strictest quality standards. Each HPLC system undergoes a series of functional tests, calibrations, and technical verifications. Components are carefully cleaned, replaced if necessary, and validated to ensure total reliability in real-world conditions.

Opting for a refurbished HPLC system offers several concrete advantages:

• Significant cost reduction compared to new, without sacrificing analytical performance.
• Rapid availability of equipment, allowing for accelerated commissioning.
• Warranty provided on every unit, with technical support if needed.
• Eco-friendly approach: giving a second life to complex equipment.

Laboratories with budgetary constraints, or those in a growth phase, can thus access reliable, tested equipment that is adapted to the requirements of the most common chromatographic analyses.

Thanks to this structured reuse model, Rewake promotes access to high performance at no extra cost, while adopting a sustainable and responsible approach.

How to choose the right HPLC system?

Choosing an HPLC system must be guided by the nature of the analyses to be performed, precision constraints, and the type of compounds to be studied. First and foremost, it is essential to determine the required level of sensitivity, the types of detection needed (UV, diode array, mass spectrometry), as well as typical sample volumes.

Here are some key criteria for selecting your equipment:

• Stationary phase: the nature of the column impacts the separation based on the polarity or size of the molecules.
• Elution type: gradient or isocratic, depending on the complexity of the mixture to be analyzed.
• Compatibility of the solvents used with the detector and column.
• Possibility of coupling with other analytical instruments like a mass spectrometer.

Rewake experts can guide you toward the HPLC system most suitable for your use: assaying organic compounds, separating isomers, purifying active substances, etc. Each technical parameter is taken into account to ensure the best configuration according to your constraints.

Delivery, installation, and after-sales service

Rewake supports its customers well beyond the purchase. All refurbished HPLC systems are shipped quickly, with secure packaging suitable for sensitive equipment. We understand the requirements of laboratories: this is why our logistics are optimized to ensure short lead times and hassle-free commissioning.

Upon request, technical assistance can be provided during installation, particularly for more complex systems requiring specific setup. We also provide maintenance, the supply of spare parts, and follow-up in the event of a malfunction.

By choosing refurbished laboratory equipment from Rewake, you benefit from a responsive and specialized after-sales service accustomed to the challenges of demanding analytical environments. All HPLC equipment is rigorously tested and covered by a warranty, for professional use with total peace of mind.

Rewake: a partner for demanding laboratories

As a specialist in refurbished laboratory equipment, Rewake has established itself as a trusted partner for organizations seeking performance, economy, and sustainability. Our expertise covers all analytical equipment, with particular attention paid to field requirements: compliance, reliability, and compatibility with existing protocols.

Laboratories face increasing budgetary and operational constraints. Rewake addresses these challenges by offering concrete, proven, and immediately available solutions. Each HPLC system offered is selected, checked, and restored according to a rigorous process, ensuring optimal analytical quality.

Choosing Rewake means relying on a partner who understands the real needs of professionals: quick access to high-performance equipment, available technical support, and transparency regarding the condition of the material and the refurbishment process.

Request a quote for a laboratory HPLC system

Are you looking for a high-performance liquid chromatography system for your analyses? Rewake offers refurbished HPLC equipment and modules. Request your quote for a laboratory HPLC system based on your analytical configuration and needs.

FAQ – HPLC: answers to frequently asked questions

What is the difference between HPLC and gas chromatography?

HPLC (high-performance liquid chromatography) uses a liquid as the mobile phase, while gas chromatography (GC) uses a carrier gas, such as helium or nitrogen. GC is suitable for volatile and thermally stable compounds, while HPLC is better suited for more complex, heat-sensitive, or low-volatility molecules. The choice depends on the nature of the solutes to be analyzed.

How does an HPLC detector work?

An HPLC detector measures the absorbance or response of an analyte after separation. The most common detectors are: UV/Vis, diode array, and mass spectrometer. They allow for the identification and quantification of compounds based on intensity variations according to wavelength or molecular weight.

Which HPLC column should I choose?

The choice of an HPLC column depends on the type of analysis: polarity of the stationary phase, particle size of the silica, mode (reversed-phase, ion-exchange, normal-phase, etc.). One must also consider the diameter, length, and compatibility with the solvents and compounds to be separated.

Can a refurbished HPLC system be used for regulatory analysis?

Yes, provided that the HPLC system is properly calibrated, validated, and maintained. Rewake's refurbished equipment is tested, cleaned, and meets the precision and reproducibility requirements necessary for standardized quantitative analyses.

How much does a refurbished laboratory HPLC cost?

The price varies depending on the brand, type of detector, age, features, and included accessories. On average, a refurbished HPLC system costs between €5,000 and €20,000, representing significant savings compared to new equipment, while ensuring an excellent level of performance.