HPLC

Accessible and reliable HPLC chromatography

High-performance liquid chromatography (HPLC) is a widely used analytical technique for separating and identifying the components of a liquid mixture. It relies on the circulation of a solvent at high pressure through a column containing a stationary phase, allowing the differentiation of molecules according to their physicochemical properties. This method is known for its accuracy, sensitivity, and ability to handle a wide variety of samples.

Rewake offers a selection of tested, ready-to-use equipment to make this technology accessible to laboratories with varying budgetary and operational constraints. The available systems meet common chromatography analysis requirements, whether for quality control, applied research, or analytical development. By using refurbished laboratory equipment, teams benefit from a reliable, immediately usable solution that complies with current standards, while minimizing the costs and time associated with acquiring new equipment.

What is the purpose of an HPLC system in a laboratory?

An HPLC system is designed to separate, identify, and quantify the different components of a complex mixture. In practice, it provides a chromatogram that highlights the compounds present, their relative concentration, and their retention time. This method is based on the principle of separation between a mobile phase (liquid) and a stationary phase (generally a porous material such as silica), in which molecules migrate at different rates depending on their chemical properties.

HPLC equipment is essential in many analytical protocols where accuracy and reproducibility are crucial. It is commonly used for:

• The dosage of active substances in medicines
• The detection of contaminants in environmental samples
• Analysis of organic constituents in complex formulations

Thanks to their compatibility with various types of detectors (UV, fluorescence, mass spectrometry), HPLC systems offer great flexibility in applications. They provide a high-performance solution for the chromatographic separation of polar or nonpolar compounds, even at low concentrations.

Applications of HPLC chromatography

HPLC chromatography is used in a multitude of sectors where the analysis of chemical substances is essential. Its high level of precision, combined with its ability to separate complex compounds, makes it an indispensable method in several scientific and industrial fields:

• Pharmaceutical industry : quality control of active ingredients, verification of purity, dosage of excipients or residual impurities.
• Cosmetics : analysis of formulation stability, detection of preservatives or sensitive molecules.
• Agri-food : detection of contaminants, additives or pesticide residues in food.
• Environment : measurement of pollutants in soils, water or ambient air.
• Analytical chemistry : characterization of complex mixtures, research on the properties of molecules and their behavior in the liquid phase.

Thanks to its numerous coupling possibilities (UV, MS, diode array, etc.), HPLC is a reference technique for quantitative and qualitative analysis. It is used for both research and regulatory analyses, with strict requirements for reproducibility and sensitivity.

HPLC equipment offered by Rewake

Rewake's HPLC collection comprises equipment designed to meet the demands of analytical laboratories, whether for research, quality control, or regulatory testing. The systems offered cover the essential needs of high-performance liquid chromatography, with configurations adapted to varying levels of analytical complexity.

Laboratories can find the following in this collection:

• Complete refurbished HPLC systems , including high-pressure pumps, injectors and control modules.
• HPLC columns adapted for the separation of polar or nonpolar compounds, depending on the stationary phase used.
• UV detectors, diode array detectors or detectors compatible with coupling to a mass spectrometer.
• Manual or automatic injectors ensuring precise and reproducible injection.

Each piece of equipment is cleaned, inspected, and tested before being offered for sale to ensure reliable operation from the moment of installation. Refurbishment allows laboratories to access high-performance equipment while controlling their budget, without compromising on analytical quality or the robustness of the instruments.

Why choose a refurbished HPLC from Rewake?

Rewake is committed to offering refurbished laboratory equipment that meets the strictest quality standards. Each HPLC system undergoes a series of functional tests, calibrations, and technical verifications. Components are thoroughly cleaned, replaced if necessary, and validated to guarantee complete reliability under real-world conditions .

Choosing a refurbished HPLC system offers several concrete advantages:

• Significant cost reduction compared to new, without sacrificing analytical performance.
• Rapid availability of equipment, allowing for accelerated commissioning.
• Warranty provided on each device, with technical support if needed.
• Eco-responsible approach: recovery and second life of complex equipment.

Laboratories with budgetary constraints, or in the process of ramping up production, can thus access reliable, tested equipment adapted to the requirements of the most common chromatographic analyses.

Thanks to this structured reuse model, Rewake promotes access to high performance without additional cost, while adhering to a sustainable and responsible approach.

How to choose the right HPLC system?

The choice of an HPLC system should be guided by the nature of the analyses to be performed, the precision requirements, and the type of compounds to be studied. Above all, it is essential to determine the required sensitivity level, the necessary detection methods (UV, diode array, mass spectrometry), and the typical sample volumes.

Here are some key criteria for selecting your equipment:

• Stationary phase : the nature of the column impacts the separation according to the polarity or size of the molecules.
• Elution type: gradient or isocratic, depending on the complexity of the mixture to be analyzed.
• Compatibility of the solvents used with the detector and the column.
• Possibility of coupling with other analytical instruments such as a mass spectrometer.

Rewake experts can guide you towards the HPLC system best suited to your use: dosage of organic compounds, separation of isomers, purification of active substances… Every technical parameter is taken into account to ensure the best configuration according to your constraints.

Delivery, installation and after-sales service

Rewake supports its customers far beyond the purchase. All refurbished HPLC systems are shipped quickly, with secure packaging suitable for sensitive equipment. We understand the demands of laboratories: that's why our logistics are optimized to guarantee short lead times and uncomplicated commissioning.

Upon request, technical assistance can be provided for installation, particularly for more complex systems requiring specific configuration. We also provide maintenance, spare parts, and troubleshooting support.

By choosing refurbished laboratory equipment from Rewake, you benefit from responsive and specialized after-sales service, experienced in the challenges of demanding analytical environments. All HPLC equipment is rigorously tested and covered by a warranty, ensuring complete peace of mind for professional use.

Rewake: Partner of demanding laboratories

As a specialist in refurbished laboratory equipment, Rewake has established itself as a trusted partner for organizations seeking performance, cost-effectiveness, and sustainability. Our expertise covers all types of analytical equipment , with a particular focus on field requirements: compliance, reliability, and compatibility with existing protocols.

Laboratories are facing increasing budgetary and operational constraints. Rewake addresses these challenges by offering concrete, proven, and readily available solutions. Each HPLC system offered is selected, tested, and refurbished according to a rigorous process, ensuring optimal analytical quality.

Choosing Rewake means relying on a partner who understands the real needs of professionals: quick access to high-performance equipment, available technical support, transparency on the condition of the equipment and on the refurbishment process.

FAQ – HPLC: Answers to Frequently Asked Questions

What is the difference between HPLC and gas chromatography?

High-performance liquid chromatography (HPLC) uses a liquid as the mobile phase, while gas chromatography (GC) uses a carrier gas, such as helium or nitrogen. GC is suitable for volatile and thermally stable compounds, while HPLC is better suited to more complex, heat-sensitive, or low-volatility molecules. The choice depends on the nature of the solutes to be analyzed.

How does an HPLC detector work?

An HPLC detector measures the absorbance or response of an analyte after separation. The most common detectors are UV/Vis, diode array, and mass spectrometer . They allow the identification and quantification of compounds based on variations in intensity according to wavelength or molecular weight.

Which HPLC column should I choose?

The choice of an HPLC column depends on the type of analysis: polarity of the stationary phase, silica particle size, mode (reverse phase, ionic, normal, etc.). The diameter, length, and compatibility with the solvents and compounds to be separated must also be considered.

Can a refurbished HPLC system be used for regulatory analyses?

Yes, provided the HPLC system is properly calibrated, validated, and maintained. Rewake's refurbished equipment is tested, cleaned, and meets the accuracy and reproducibility requirements necessary for standardized quantitative analyses.

How much does a refurbished laboratory HPLC cost?

The price varies depending on the brand, detector type, age, features, and included accessories. On average, a refurbished HPLC system costs between €5,000 and €20,000, representing significant savings compared to a new one, while still guaranteeing excellent performance.