The laboratory centrifuge is an essential piece of equipment in many scientific fields. From molecular biology to medicine to food processing, it allows you to separate the components of a mixture based on their density. But its use cannot be improvised: improper handling can compromise your results, damage the device, or endanger users. In this article, discover how to optimally use a laboratory centrifuge , step by step.
Why use a laboratory centrifuge?
The purpose of a laboratory centrifuge is to separate substances in a mixture based on their density by the action of centrifugal force. This allows in particular to:
- Separate cells and fluids in medical analyses.
- Purify macromolecules (DNA, proteins, viruses).
- Extract organic or aqueous phases in chemistry.
- Clarify suspensions or remove cell debris.
Preparing your laboratory centrifuge
1. Inspection before use
Before using a laboratory centrifuge , check that:
- The rotor is clean, without cracks or chemical residue.
- The room is free from dust or projections.
- The tubes are compatible with the model and in good condition.
2. Balancing the samples
This is the crucial step. Poor weight distribution can damage the rotor or lead to failure.
- Always use identical tubes, filled to equal volumes.
- Place the tubes in perfect symmetry with respect to the rotor axis.
3. Setting the parameters
Settings vary depending on the sample type:
- Speed (in revolutions per minute, RPM or relative centrifugal force, RCF)
- Centrifugation time
- Temperature (for refrigerated centrifuges)
How to use a laboratory centrifuge safely?
1. Loading
Carefully insert the tubes into the rotor cavities. Make sure the cover is securely locked before starting the unit.
2. Start of centrifugation
Press the start button, then stay nearby for the first few seconds. Any abnormal vibration or noise should alert you immediately.
3. End of the cycle
Wait for the rotor to come to a complete stop before opening the cover. Never attempt to stop it manually.
Routine Maintenance of the Laboratory Centrifuge
- Clean the rotor after each use with a soft cloth and non-abrasive detergent.
- Dry the compartments completely before closing the centrifuge.
- Schedule a complete annual maintenance.
Mistakes to avoid
- Using cracked or incompatible tubes.
- Unbalanced loads or incorrectly positioned tubes.
- Forgetting to lock the lid.
- Using the device with volatile substances without precautions.
Rewake: an economical and reliable solution for your centrifugation equipment
At Rewake, we meticulously recondition every laboratory centrifuge . Each device is:
- Tested to current safety and performance standards.
- Cleaned, calibrated and checked by specialized technicians.
- Guaranteed for immediate and long-lasting use.
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Practical applications of the laboratory centrifuge
In a modern laboratory, the laboratory centrifuge is used in a wide variety of contexts. In biology, it is used to separate blood components (plasma, red blood cells, white blood cells), concentrate cells, or purify DNA and RNA. In analytical chemistry, it is used to isolate solid particles after a reaction. In the food industry, it helps separate liquid phases of ingredients or test the stability of certain products.
It is therefore far from limited to academic or hospital use. Many industrial laboratories use it daily for their quality control, formulation, or research and development processes. This is why understanding the correct usage procedures becomes essential, regardless of your specialty.
Compliance with standards and safety in the laboratory
Using a laboratory centrifuge also requires compliance with certain standards. First, it is crucial to verify that the equipment complies with applicable CE directives and ISO standards (e.g., ISO 61010 for electrical laboratory equipment). These certifications ensure optimal safety of use, both for the user and for the integrity of the samples.
When it comes to safety, certain reflexes must become systematic: always balance loads, check that the lid is properly locked, and never open the device before it has completely stopped. In addition, wearing gloves, goggles, and a lab coat is recommended, especially when processing high-risk biological or chemical samples.
Integration into laboratory protocols
A laboratory centrifuge is never used alone: it is part of a larger workflow. It is often preceded by a pipetting or sample preparation step, and followed by analysis on a spectrophotometer, plate reader, or microscope. For this reason, it must be positioned in a convenient environment, close to other instruments, without obstructing circulation on the bench.
Good organization not only streamlines handling, but also limits the risk of cross-contamination or dosing errors. Also consider providing a dedicated space for centrifugation accessories: rotors, adapters, replacement tubes, etc.
Recommendations for investing wisely in your equipment
Finally, if you're just starting to equip your lab or replacing an old machine, it's essential to take a long-term view. Rather than focusing solely on price, consider the equipment's durability, the availability of spare parts, and the quality of after-sales service.
At Rewake, each refurbished laboratory centrifuge is rigorously tested, inspected, and calibrated to deliver excellent performance at a lower cost. You benefit from reliable equipment, backed by a warranty, while adopting an eco-responsible approach.
Mastering the use of a laboratory centrifuge isn't just about its technical operation. It's also about understanding its role in an overall process, following best safety practices, and optimizing its use in daily laboratory operations. To support this skill development, the Rewake team remains available to advise you on the model best suited to your activity.
