What is a micropipette pipette?
The micropipette pipette is a volumetric measuring instrument used to aspirate and transfer very small volumes of liquids, ranging from a few microliters to several milliliters depending on the model. It is essential in molecular biology, analytical chemistry, pharmacology, and more generally in any laboratory requiring precise dosages.
It works thanks to a piston system that creates a vacuum to draw the liquid into a disposable tip (or tip) adapted to its volume. This mechanism guarantees rapid, sterile and reproducible handling, essential to avoid any contamination or experimental error.
The different types of micropipettes
There are several large families of micropipette pipettes adapted to each type of application:
Fixed volume micropipettes
Perfect for repetitive protocols, they always aspirate the same volume. They offer excellent precision and are often used in PCR, sequencing, or ELISA testing techniques.
Variable volume micropipettes
These models allow you to adjust the volume directly on the pipette. Ideal for laboratories that handle varying volumes without changing instruments.
Multichannel micropipettes
Composed of 8 to 12 parallel channels, they allow you to pipette several samples simultaneously, very useful for filling 96-well plates during high-throughput analyses.
How to use a micropipette pipette correctly?
Proper use of the micropipette pipette is essential to ensure accuracy and repeatability:
- Choose the tip that matches the volume range of your pipette.
- Suck in and out the liquid slowly to avoid bubbles forming.
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Keep the pipette vertical when aspirating to ensure accurate volume.
- Change the tip after each sample to avoid cross-contamination.
- Follow the calibration instructions depending on the frequency of use.
These simple steps guarantee reliable results, especially in sensitive experiments such as PCR or enzymatic assay.
Why is precision crucial in the laboratory?
Every microliter counts in molecular biology, analytical chemistry, or pharmacology protocols. Even a small error during pipetting can lead to:
- False positives or false negatives in the analyses.
- Significant variability in experimental results.
- Waste of time and expensive reagents .
This is why it is essential to choose a quality laboratory pipette and to follow rigorous pipetting procedures on a daily basis.
What criteria should you use to choose your micropipette?
Before purchasing your micropipette pipette, several criteria must be analyzed:
- The volume range suited to your needs (e.g. 0.5-10 µL, 20-200 µL, 100-1000 µL).
- The precision and accuracy specified by the manufacturer.
- Compatibility with universal tips or specific tips.
- Ergonomics : weight, pipetting comfort, volume adjustment system.
- Ease of maintenance and calibration .
At Rewake, we offer tested, calibrated and reconditioned models, guaranteeing the same precision as a new pipette, for an optimized budget.
Micropipette pipette price: how much does this essential tool cost?
The price of a micropipette pipette depends mainly on the type of instrument, its brand, its features and its condition (new or reconditioned):
- Fixed volume micropipette : from €60 new, around €30 reconditioned.
- Variable volume micropipette : between €100 and €400 depending on the precision required.
- Multi-channel micropipette : from €500 to €1200, depending on the number of channels and the technology used.
By choosing a reconditioned micropipette from Rewake, you benefit from certified equipment at much more affordable prices, without sacrificing either precision or reliability.
Buying a micropipette: how to choose the right one?
To help you make the most of your micropipette purchase , here are some practical tips:
Analyze your specific needs
Ask yourself the right questions: Are you working with small volumes (1-10 µL) or larger samples (200-1000 µL)? Do your projects require a single or multi-channel pipette for 96-well plates?
Compare the advertised performances
Check the model datasheets: systematic errors (accuracy) and random errors (precision) are key performance indicators to compare.
Think about ergonomics and comfort
An ergonomic micropipette reduces fatigue during long series and improves the reproducibility of your manipulations.
Maintenance and calibration of a micropipette pipette
A poorly maintained pipette can ruin all your experiments. To extend the life of your instrument:
- Clean the exterior regularly with isopropyl alcohol.
- Change the gaskets according to the frequency of use.
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Have your micropipette calibrated at least once a year by an accredited laboratory.
At Rewake, our refurbished models are systematically tested and recalibrated before being offered for sale, to guarantee optimal performance upon receipt.
Micropipette pipette: the reference brands
On the market, some brands stand out for their quality and reliability:
- Eppendorf : essential in biology and chemistry laboratories.
- Gilson : recognized for its robustness and ease of use.
- Sartorius : specialist in ergonomic and precise micropipettes.
- Thermo Fisher Scientific : offers a wide range to suit all budgets.
Rewake only selects micropipettes from major brands, verified and guaranteed, to offer laboratories the best quality/price combination.
Micropipette pipette and good pipetting practices
Here are some expert tips to maximize precision during your manipulations:
- Always use tips that are appropriate for your model and volume range.
- Respect temperatures : Pipette liquids and use tips at room temperature to avoid volume variations.
- Perform a pre-aspiration (aspirate/expel once before taking a sample to homogenize the pressure).
- Pipette slowly to avoid air bubbles.
Pipetting training and practical experience are just as essential as the quality of the instrument itself to achieve reliable results.
Why invest in a quality micropipette pipette?
The micropipette is a central tool in many scientific disciplines. A reliable and well-maintained model ensures the accuracy of your assays, the reproducibility of your results, and the protection of your samples.
At Rewake, we offer a wide selection of reconditioned laboratory pipettes, combining technical performance with significant savings. Discover our laboratory pipette catalog now to equip your lab with certified professional equipment.
Don't let poor pipetting compromise your research: equip yourself with the best equipment, calibrated and ready to use!
The accuracy of your assays starts with the right choice of micropipette , whether manual or electronic. To further analyze your samples, also explore our selection of microscopes , designed to meet the demands of modern laboratories.
